Design, develop, and execute functional genomics assays, including CRISPR-based editing (e.g., RNP delivery, Perturb-seq), single-cell sequencing, spatial transcriptomics, and CITE-seq.
Provide technical guidance and training to research scientists on FGSL technologies and techniques, including assay execution, troubleshooting, and resolution of technical issues;
Documents and maintains detailed laboratory records in accordance with policies;
Operates and maintains lab equipment in collaboration with other lab members;
Assists acquisition of necessary supplies and equipment;
Defines and performs appropriate QC measures;
Follows relevant scientific literature to ensure use of optimal methods and understand emerging practices across the field;
Demonstrates the ability to interpret the outcome of experiments, propose appropriate follow-up, and may propose new avenues of investigation;
Communicates own work effectively orally and in writing; contributes to writing protocols, procedures, and technical reports
Automate processing and results reporting and delivery;
Reports and treats data with a high level of integrity and ethics
Required Experience:
CRISPR assays - Must have personally designed guides, performed transfections/infections, selected edited cells, and validated knockouts/knockins
Next-generation sequencing (NGS) - Hands-on library preparation experience (not just data analysis)
Single cell technologies - Personal experience with sample prep, library generation, and quality control
Standard molecular biology techniques - qPCR, Western blot, cloning, gel electrophoresis
Cell culture - Proficient in maintaining multiple cell lines, transfections, and sterile technique
Experience in automation is a plus;
Able to perform variety of FGS experimental techniques at the same time;
Demonstrated experience working in a multi-disciplinary and collaborative environment;
Ability to troubleshoot FGS experiments both individually and as part of a team.
Excellent oral and written skills with the ability to communicate in an open, transparent, timely and consistent manner
Education Required:
Ph.D. or Master’s degree from an accredited institution with 6+ years of experience in molecular, cell biology, or related scientific discipline.
